Nphs2^{tm1.1(icre)Ptb}

The targeting construct designed to insert a codon improved Cre Recombinase gene (icre) and a membrane-targeted tandem dimer tdTomato (mTomato) under the control of the endogenous Nphs2 promoter using viral T2A-peptides was generated using bacterial artificial chromosome clones from the C57BL/6J RPCIB-731 BAC library. The T2A.icre.T2A.mTomato cassette was inserted between the last codon of exon 8 of Nphs2 and the stop codon. A frt-flanked Puromycin resistance cassette was placed in intron between exon 7 and exon 8. The constitutive knock-in allele was generated after Puromycin resistance cassette was removed by in vivo Flp-mediated removal of the selection marker. (J:264681)