Patl2^{tm1b(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 121956938 of Chromosome 2 upstream of the exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 7 at position 121957459. Exon 7 is thus flanked by loxP sites. Cre-mediated recombination removed exon 7 creating a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtm (J:157065, J:264835)