The transposon containing an unspecified hybrid actin promoter driving expression of red fluorescent protein (RFP) flanked by piggyBac-specific left and right terminal sequences inserted at position 55,036,508 (Ensemble version 85) in intron 7. Western blot analysis confirmed the absence of protein in brains from E12.5 homozygous embryos. (J:123680, J:262219)