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A cDNA encoding a MerCreMer fusion protein and an FRT-flanked neomycin cassette were recombined in-frame with the Ly6a ATG start codon (exon 2) via homologous recombination in embryonic stem (ES) cells. The MerCreMer double fusion protein incorporates a cre recombinase cDNA sequence flanked by mutated murine estrogen receptor (mer) ligand binding domains (amino acids 281-599, G525R). Resultant mice were crossed with Flpe mice to delete the neomycin cassette. Expression of Ly6a is eliminated in the targeted allele. (J:264764)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
