Akap5^tm3.1Mdaq

The Akap5CS mutation eliminates palmitoylation of AKAP150 by mutating cysteines 36 and 123 to serines. These mutations were introduced into the single coding exon of an Akap5 genomic DNA fragment via piggyBac (PB) transposon based method from a C57BL/6 BAC clone. In this targeting vector, the CS mutation and a C-terminal myc-epitope tag were introduced by piggyBac method with a neomycin resistance cassette flanked by the 3 and 5 long-terminal repeat (LTR) of PB inserted within the Akap5 exon. The neomycin resistance cassette was then removed from the targeted locus by remobilizing the PB with transient expression of PB transposase. (J:269375)