Trem2^em2Bwef

The allele was generated by injecting cas9 nuclease and a single guide RNA designed to introduce an arginine to histidine (R27H) point mutation with three silent mutations GA>TC to create a restriction enzyme site for genotyping and G>A to block the protospacer-adjacent motif to allow higher editing efficiency. Western blot of microglia and bone marrow derived macrophages confirms a reduction in protein levels. (J:264639)