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A targeting construct was designed to replace the stop codon of the growth hormone releasing hormone (Ghrh) gene with a viral P2A oligopeptide, from porcine teschovirus-1, fused to a Cre recombinase gene. A frt-flanked neomycin resistance (neo) cassette inserted downstream. The construct was electroporated into R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.129S4-Gt(ROSA)26Sor/J mice to remove the neo cassette. (J:264547)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
