Slc30a8^tm1.1Vlcg

Nucleotide 409 was changed from a C to T in exon 3 and a self-excising neo cassette was inserted in intron 3 deleting 23 bp of sequence via homologous recombination. The mutation in exon 3 changes an arginine into a stop codon. RNA in situ hybridization showed a 70% reduction in mRNA expression in pancreatic islets from homozygous mice. However, western blot analysis failed to detect any protein in this tissue in homozygous mice. This mutation is analogous to the human p.Arg138* variant. (J:264368)