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Cas9 mRNA and gRNA (5'-AGCATCCTGGCATTCCGAG-3') were co-injected into C57BL/6NCrl zygotes. Founders with frameshift mutations were screened via T7E1 assay and validated by DNA sequencing. This mutation carries an 37 bp deletion in exon 5 of the gene. (J:226781)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
