Tg(Acta2-PTHLH)375Clem

a 3.6-kb fragment of the mouse smooth muscle alpha actin gene (SMP8) containing 1074 bp of the upstream (promoter) region, the 63 bp exon 1 (5' UTR), the 2.5 kb first intron, and 15 bp of exon 2 5' UTR), was used to drive the expression of the human parathyroid hormone-like peptide cDNA sequence. SV40 poly(A) signal sequences (240 bp) are placed downstream of the cDNA. Northern blot experiments showed very high levels in bladder, stomach, uterus, and aorta and to lower expression in intestine. In situ hybridization showed that the expression was restricted to smooth muscle, including the smooth muscle cell layers of the intestine, aorta, bladder, stomach, bronchus, pulmonary artery, uterus, and meningeal and renal arterioles. (J:54258)