Arc^tm2.1Grnt

Sequence coding for a TAP (Tandem Affinity Purification) tag (containing Histidine Affinity Tag (HAT), TEV protease and 3x Flag sequences), a linker (coding for GGGS), and the Venus yellow fluorescent reporter, followed by a loxP site flanked neomycin resistance gene cassette, was inserted before the translation stop codon in exon 1. The neo cassette was removed through subsequent cre-mediated recombination. This leaves an allele coding for a chimeric protein of the endogenous protein with a C-terminal TAP tag and Venus peptide. (J:254217)