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The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the transmembrane protein 119 locus (Tmem119) on chromosome 5. The cas9-RNP complex and a 2A-CreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the CreER fusion gene with ~1.5 kbp homology arms were injected into the pronucleus of C57BL/6NTac mouse embryos. Founders were bred to C57BL/6J mice for germline transmission. (J:280123)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
