Tg(RNU6-1-RNAi:Cdk5)#Chad

The human U6 small nuclear RNA gene promoter was used to drive expression of a mouse anti-Cdk5 shRNA. The construct was microinjected into the male pronuclei of C57BL/6J zygotes. Three founders were generated. Immunohistochemical and Western blot analyses confirmed that protein expression was decreased in the skin of knockdown mice relative to wild-type controls. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:255707)