Gt(ROSA)26Sor^{tm1(CAG-EGFP)Brsy}

The targeting construct containing (from 5' t 3') CAG-loxP-neomycin (reversed)-pA-loxP-3xHA-EGFP-SYNJ2BP-lacZ-BGH-pA is targeted to intron 1 of the gene. The 3xHA-EGFP-SYNJ2BP components constitute the "MITO-Tag" cassette targeting the 3XHA epitope tag with GFP to the mitochondrial membrane. The construct includes only the transmembrane domain of the rat Synj2bp/OMP25 (AA109-145) gene. The small fragment of lacZ incorporated in the mutation is not expected to be functional. (J:268121)