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PCR primers with flanking restriction sites were designed to amplify the rearranged Valpha3.1Jalpha40 and Vbeta2.1Jbeta2.1 segments from genomic DNA of the Ob1A12 T cell clone. These DNA segments were then subcloned into murine TCR-specific regulatory elements containing genomic TCR expression cassettes pTalphaCass and pTbetaCass, respectively, and microinjected into fertilized oocytes. Mice with this transgene express human anti-MBP (myelin basic protein) 85-99/DR15 T cell receptors. (J:90756)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
