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The second coding exon was flanked by loxP sites. An frt-flanked neomycin selection cassette was inserted between the loxP site and the 5 end of exon 2. Exon 2 and the neomycin selection cassette was removed by Cre-mediated recombination. Quantitative PCR analysis of homozygous mutant mouse mRNA in cortex and spinal cord confirmed the absence of exon 2 containing mRNA. Western blot analysis of brain extracts of homozygous mice indicated that no detectable encoded protein was present. (J:263474)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
