A loxP site and a an FRT site flanked puromycin resistance gene cassette were inserted into intron 2 and a second loxP site into intron 3. The puro cassette was removed through flp-mediated recombination, leaving a conditional-ready allele with exon 3 floxed. (J:261991)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count