Hoxc^tm1.1Ssp

The BAC BMQ257-D8 was used to sequentially targeted Hoxc9, Hoxc10, and Hoxc11. In all three genes a kan/neo cassette flanked by once only lox66 and lox71 was introduced into exon 1. This insertion introduced a small deletion/frameshift. In Hoxc9 and Hoxc10 the selection cassette was removed by cre mediated recombination before targeting of the subsequent gene. Thus only Hoxa11 retains the selection cassette in the final targeting vector and this was removed after targeting by cre mediated recombination. (J:262635)