Exon 2 was flanked by loxP sites and a neomycin cassette and a third loxP site were inserted downstream of the floxed exon 2. The selection cassette was excised by transiently transfecting ES cells with a cre recombinase-expressing construct. (J:262526)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count