Tg(hs840-cre/ERT2,-GFP)75Jlr

This transgene contains the human enhancer element hs840 -- which is flanked on human Chr 4 by the EPHA5 [EPH receptor A5] antisense RNA 1 (EPHA5-AS1) and Homo sapiens uncharacterized LOC101927237 long non-coding RNA (LOC101927237) loci -- followed by a mouse minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus. (J:214335)