A loxP site was inserted upstream of exon 1 and a FRT-flanked PGK-gb2-neo cassette in opposite coding direction followed by a second loxP site were inserted downstream of exon 1. Flp-mediated recombination removed the neomycin selection cassette, leaving exon 1 floxed. (J:246647)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count