A loxP site, FRT flanked pgk-neo construct, tetO construct and loxP site were inserted upstream of the translation initiation start site via homologous recombination. Flp mediated recombination removed the neo cassette. (J:261716)
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A loxP site, FRT flanked pgk-neo construct, tetO construct and loxP site were inserted upstream of the translation initiation start site via homologous recombination. Flp mediated recombination removed the neo cassette. (J:261716)