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The reporter cDNA is introduced to mouse Igk regulatory elements: Vkappa21E promoter-noncoding leader (L) exon-Vkappa exon-mNeonGreen/mRuby2 (INDIA) cDNA-polyA-Jkappa5 intronic sequence with enhancer (ie) and 3' regulatory region (rr). The reporter consists of an optimized fluorescence (Forster) resonance energy transfer (FRET) pair: N-terminally Myc-tagged mNeonGreen and mRuby2, linked by an 18 amino acid peptide containing an aCasp3-cleavage site (DEVDG). When aCasp3 (Casp3, caspase 3; a marker for apoptosis) cleaves the linker, mNeonGreen and mRuby2 are separated, resulting in FRET loss and increased emission from mNeonGreen. Non-apoptotic cells retaining uncleaved mNeonGreen/mRuby2 demonstrate more yellow fluorescence indicating a lower ratio of mNeonGreen/FRET fluorescence emission. (J:261534)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
