Hmces^em2Dfel

Cas9 mRNA and single-guide RNA (sgRNA) targeting exon 2 was microinjected into the cytoplasm of zygote-stage embryos to induce deletions. Injected embryos were implanted in CD-1 foster mice and pups screened for deletions. Two lines were generated. This allele has a 71 bp deletion encompassing the translation initiation codon and catalytic invariant Cys2 codon. (J:254234)