A loxP site was inserted upstream of exon 3 and a FRT-flanked PGK-EM7-Neo selection cassette and a second loxP site were inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette leaving exon 3 floxed. (J:260823)
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A loxP site was inserted upstream of exon 3 and a FRT-flanked PGK-EM7-Neo selection cassette and a second loxP site were inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette leaving exon 3 floxed. (J:260823)