Sp9^tm1.2Zyan

A targeting vector was designed to insert a FRT site followed by an splice acceptor (SA) fused to the internal ribosomal entry site (IRES) and a lacZ gene, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) intron 1 and a third loxP site immediately downstream of the exon2. Flpe-mediated recombination removed reporter and neo cassette from Sp9tm1Zyan leaving a single FRT site in intoron 1 and loxP flanked exon 2. This allele is a conditional-ready allele. (J:238868)