A loxP site was inserted downstream to exon 4 and a loxP-frt-flanked Neomycin selection cassette was inserted in intron 3, upstream of exon 4. The neomycin gene was removed via Flp-mediated recombination. (J:259798)
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A loxP site was inserted downstream to exon 4 and a loxP-frt-flanked Neomycin selection cassette was inserted in intron 3, upstream of exon 4. The neomycin gene was removed via Flp-mediated recombination. (J:259798)