Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy

The transgene was designed to have (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, EGFP, a transcriptional/translational STOP cassette (containing a PGK-neo), a second loxP site, a mouse cDNA sequence encoding precursor SUMO1 with N-terminal histidine tag (His-SUMO1), a viral 2A oligopeptide sequence (P2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO2 with N-terminal hemagglutinin tag (HA-SUMO2), a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO3 with N-terminal FLAG tag (FLAG-SUMO3), a viral 2A oligopeptide sequence (F2A; mediates ribosomal skipping), the monomeric DsRed fluorescent protein variant mCherry gene and a bovine growth hormone polyadenylation signal. Eight founder lines were created, founder line 10 exhibits ubiquitous expression of GFP. (J:260338)