A loxP aite was inserted 5' to exon 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 1. Cre-mediated recombination removed the loxP-flanked neomycin selection cassette, leaving a single loxP site 3' to exon 1. (J:259087)
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A loxP aite was inserted 5' to exon 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 1. Cre-mediated recombination removed the loxP-flanked neomycin selection cassette, leaving a single loxP site 3' to exon 1. (J:259087)