A loxP site was inserted upstream of exon 2 and FRT-flanked neomycin cassette followed by a second loxP site were inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette, leaving exon 2 floxed. (J:246056)
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A loxP site was inserted upstream of exon 2 and FRT-flanked neomycin cassette followed by a second loxP site were inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette, leaving exon 2 floxed. (J:246056)