Exon 1 was replaced with an IRES-rtTA expression cassette followed by an frt-flanked neomycin resistance cassette. FLP-mediated recombination removed the neomycin selection cassette. (J:259125)
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Exon 1 was replaced with an IRES-rtTA expression cassette followed by an frt-flanked neomycin resistance cassette. FLP-mediated recombination removed the neomycin selection cassette. (J:259125)