A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 3. The neomycin selection cassette was removed via FLP-mediated recombination. This creates a conditional-ready allele with exons 2 and 3 floxed and without the neomycin selection cassette. (J:241981)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count