A 1.6-kb DNA fragment containing exon 2 was flanked with loxP sites, with an frt-flanked neomycin resistance cassette inserted downstream of exon 2. The neomycin selection cassette was removed by crossing to Flp mice. (J:236134)
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A 1.6-kb DNA fragment containing exon 2 was flanked with loxP sites, with an frt-flanked neomycin resistance cassette inserted downstream of exon 2. The neomycin selection cassette was removed by crossing to Flp mice. (J:236134)