A loxP site and FRT flanked selection cassette were inserted upstream of exon 3 and an F3 site and loxP site were inserted downstream of exon 3 via homologous recombination. Flp meditated recombination removed the selection cassette. (J:147112)
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A loxP site and FRT flanked selection cassette were inserted upstream of exon 3 and an F3 site and loxP site were inserted downstream of exon 3 via homologous recombination. Flp meditated recombination removed the selection cassette. (J:147112)