A FRT-flanked PGK-neomycin cassette and a loxP site were inserted upstream of exon 3 and a second loxP site downstream of exon 4. Flp-mediated recombination removed the selection cassette, leaving exons 3-4 floxed. (J:257972)
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A FRT-flanked PGK-neomycin cassette and a loxP site were inserted upstream of exon 3 and a second loxP site downstream of exon 4. Flp-mediated recombination removed the selection cassette, leaving exons 3-4 floxed. (J:257972)