Exons 5 and 6 were flanked by loxP sites and a FRT-flanked positive selection marker puromycin was inserted in intron 4. Flp-mediated recombination removed the puromycin gene. (J:247100)
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Exons 5 and 6 were flanked by loxP sites and a FRT-flanked positive selection marker puromycin was inserted in intron 4. Flp-mediated recombination removed the puromycin gene. (J:247100)