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A loxP site and FRT-flanked pgk-puro cassette were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 4 via homologous recombination. Cre-mediated recombination removed the pgk-puro cassette and exons 2-4. RT-PCR and western blot analyses confirmed the absence of expression in testes from homozygous mice at 8 weeks of age. (J:251285)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
