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A cassette bearing a tdTomato/T2A/Cre-ERT2/bGH polyA was inserted in frame with the mouse Mex3a start codon in exon 1. After homologous recomibination in ES cells, positive clones were expanded and then transfected with an FlpO-bearing plasmid to remove the neomycin cassette. Positive clones were selected and blastocyst injection was done to generate chimeras. The chimeras with successful germline transmission were bred with C57BL6 mice and the offspring was used to generate the final mouse colonies. (J:253826)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
