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A FRT-flanked trapping element consisting of a splice acceptor sequence followed by a neomycin resistance gene and a loxP site were inserted into introns 1-2. A second loxP site was inserted into introns 8-9 so that exons 2-8 were floxed. qRT-PCR analysis demonstrated the loss of mRNA expression in liver, muscle and bone marrow tissue from homozygous mutant mice. Western blot analysis confirmed absence of encoded protein. (J:233169)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
