A loxP site was inserted upstream of exon 2 and a floxed neo cassette was inserted downstream of exon 2 via homologous recombination. Cre-mediated recombination removed the neo cassette leaving exon 2 floxed. (J:256240)
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A loxP site was inserted upstream of exon 2 and a floxed neo cassette was inserted downstream of exon 2 via homologous recombination. Cre-mediated recombination removed the neo cassette leaving exon 2 floxed. (J:256240)