A loxP site was inserted upstream of exon 1 and a construct, containing an FRT site V6.5, polyA sequence, loxP site, neo cassette, FRT site and loxP site, was inserted downstream of exon 2 via homologous recombination. Flp-mediated recombination removed the polyA sequence and neo cassette leaving exons 1 and 2 floxed. (J:256465)
Basic Information
(C57BL/6 x 129S4/SvJae)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count