Tg(Plp1-Gal3st1)2645Gie

The transgenic construct contains (from 5' to 3') a ~12.7 kbp promoter/enhancer sequence from the mouse proteolipid protein (myelin) 1 locus, a ~1.3 kbp cDNA sequence encoding mouse galactose-3-O-sulfotransferase 1 and an SV40 polyA sequence. The PLP fragment includes its 5' flanking DNA, exon 1, intron 1 and partial exon 2 sequences, with mutations introduced into the exon 1 ATG start codon and an exon 2 ATG (to allow protein translation to start at the appropriate ATG of the inserted CST cDNA - while keeping PLP exon/intron consensus sequences that are necessary for proper splicing), as well as deletion of the first 13 amino acids of exon 2. Female founder tg2645 has a 200-fold increased Gal3st1 enzyme activity in brain homogenates (4-6 months old) compared to wildtype controls. (J:124942)