Exons 4 and 5 (the third and fourth coding exons) were flanked by loxP sites. A FRT-flanked neomycin resistance gene was inserted into the 3' region downstream of the gene. Flp-mediated recombination removed the neomycin cassette. (J:234296)
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Exons 4 and 5 (the third and fourth coding exons) were flanked by loxP sites. A FRT-flanked neomycin resistance gene was inserted into the 3' region downstream of the gene. Flp-mediated recombination removed the neomycin cassette. (J:234296)