A loxP site was inserted upstream of exon 3 and a FRT flanked PGK-promoter driven neomycin resistance gene and loxP site were inserted downstream of exon 3. Flp-mediated recombination removed the neomycin cassette leaving exon3 floxed. (J:248833)
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A loxP site was inserted upstream of exon 3 and a FRT flanked PGK-promoter driven neomycin resistance gene and loxP site were inserted downstream of exon 3. Flp-mediated recombination removed the neomycin cassette leaving exon3 floxed. (J:248833)