Chchd10^em5Lutzy

CRISPR/cas9 endonuclease mediated genome editing was used to introduce a single nucleotide insertion of an A residue within exon 2. This mutation generates a frameshift that results in premature stop codon, creating a knock-out allele. No gene product (protein) is detected by western blot analysis of brain, skeletal muscle and heart tissue from homozygous animals. (J:254726)