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The initial targeting event inserted loxP site 3 to the endogenous exons 14-15, a synthetic poly(A) site preceding a FRT-Neo-FRT cassette, a second loxP site, and a second copy of exon 14 (the last coding exon of the gene) fused to AcGFP sequence. FLPmediated recombination removed the neo selection cassette, and Cre-mediated recombination removed the endogenous exons 14 and 15, leaving the second copy of exon 14 (the last coding exon of the gene) fused to AcGFP sequence. (J:250402)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
