Col13a1^tm4.1Pih

Homologous recombination was used to insert a selection cassette flanked by loxP sites in the first intron and an RRRR- to ATAA- mutation in exon 2 to delete a furin type proprotein convertase recognition sequence. Cre-mediated recombination removed the selection cassette. This mutation results in all protein being of the transmembrane form. Western blot confirmed the presence of transmembrane forms and absence of the shed form of the protein. (J:242846)