Depdc5^em1Pqt

CRISPR/Cas9 mediated mutagenesis created a 22 base pair deletion (c77_99del/L23fsX65) resulting in a frameshift in exon 2. CRISPR guides target 5'-TGATGTGAGGAAATACTTT-3' and 5'-GTATTTCCTCACATCAAACT-3'. No difference in transcript level compared to wild-type is seen, however mass spectrometry of parallel reaction monitoring confirmed absence of DEPDC5 peptides indicating a null allele. (J:256299)