A loxP site was inserted upstream of exon 6. An FRT- and loxP-flanked neomycin resistance cassette was inserted downstream of exon 7. Flp-mediated recombination removed the selection cassette and left exons 6 and 7 floxed. (J:256244)
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A loxP site was inserted upstream of exon 6. An FRT- and loxP-flanked neomycin resistance cassette was inserted downstream of exon 7. Flp-mediated recombination removed the selection cassette and left exons 6 and 7 floxed. (J:256244)