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This allele is generated using somatic cell nuclear transfer (SCNT). Individual (C57BL/6 x 129)F1 donor nuclei from iNKT (invariant natural killer T) cells were isolated and used to replace the mitotic spindle from mouse oocytes. Cells are developed to the blastocyst stage and used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. The Tcrb allele selected for propagation from the transferred nuclei had a productive, in-frame recombination of the Vbeta 8.2 gene segment and a unique CDR3 sequence (CASSDRGYEQYF). (J:254145)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
